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|Title: ||Glutamate production by cultured microglia: differences between rat and mouse, anhancement by lipopolysaccharide and lack effect of HIV coat protein GP120 and depolarizing agents|
|Authors: ||Patrizio, Mario|
|Issue Date: ||1994|
|Publisher: ||Elsevier Scientific Publishers Ireland|
|Citation: ||Patrizio M, Levi G. Glutamate production by cultured microglia: differences between rat and mouse, anhancement by lipopolysaccharide and lack effect of HIV coat protein GP120 and depolarizing agents. Neuroscience letters 1994;178:184-188. |
|ispartof: ||Neuroscience letters|
|Peer reviewed: ||yes|
|Abstract: ||Da MEDLINE: Glutamate release from rat and mouse microglia subcultures grown in a serum-free medium was substantially greater in the presence than in the absence of a physiological concentration of glutamine (0.5 mM). Mouse microglia produced and released more glutamate than rat microglia. Glutamate accumulation in the medium increased with time and cell density, which is consistent with the virtual absence of glutamate reuptake. Lipopolysaccharide (LPS; 10-100 ng/ml), HIV coat protein gp120 (0.1-10 nM), high K+ (35 mM) or ATP (150 microM), did not affect glutamate release from cells maintained in serum-free medium. In the presence of 1% dialyzed serum, however, LPS induced a dose- and time-dependent increase in the accumulation of glutamate in the medium, suggesting that, as in other cell types, serum factors are required for LPS binding to its receptors |
|Appears in Collections:||6 - Pubblicazioni su serie non edite da ISS|
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